A comparative analysis of immunohistochemistry and fluorescent in situ hybridization assay to detect anaplastic lymphoma kinase status in lung adenocarcinoma cases: (Record no. 82597)

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fixed length control field 02903nab a22003617a 4500
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fixed length control field 171221b2017 xxu||||| |||| 00| 0 eng d
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Wagle PB
Relator term Corresponding author
245 ## - TITLE STATEMENT
Title A comparative analysis of immunohistochemistry and fluorescent in situ hybridization assay to detect anaplastic lymphoma kinase status in lung adenocarcinoma cases:
Remainder of title A search for a testing algorithm
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc 2017
300 ## - PHYSICAL DESCRIPTION
Extent
500 ## - GENERAL NOTE
General note Address for correspondence: wagalepranjali@yahoo.in
520 ## - SUMMARY, ETC.
Summary, etc INTRODUCTION:
Testing for echinoderm microtubule-associated protein-like 4 (EML4) anaplastic lymphoma kinase (ALK) translocation by fluorescence in situ hybridization (FISH) is well established whereas the Food and Drug Administration (FDA) ALK immunohistochemical (IHC) test is relatively new.

AIMS AND OBJECTIVE:
The aim of this study is to compare FDA-approved ALK IHC test (D5F3 clone) with the standard ALK FISH test.

MATERIALS AND METHODS:
A validation and a test arm with 100 and 200 cases of Formalin-Fixed, Paraffin-embedded blocks of lung adenocarcinoma, respectively, comprised the material. All cases had ALK IHC test on automated Ventana Benchmark XT IHC slide stainer using anti-ALK D5F3 rabbit monoclonal primary antibody; when positive tumor cells (any percentage) showed strong granular cytoplasmic staining. For the FISH test, Vysis ALK Dual Color Break Apart Rearrangement Probe (Abbott Molecular Inc.,) was used to detect ALK gene 2p23 rearrangements; when positive the red and green signals were split two signal diameter apart and/or isolated 3'red signal were detected in more than 15% tumor cells. The ALK FISH results were available in all 100 validation cases and 64-test arm cases which formed the basis of this analysis.

RESULTS:
The ALK IHC test was positive in 16% cases; four discordant cases were ALK IHC positive but ALK FISH negative, but no case was ALK IHC negative and ALK FISH positive. There was 100% sensitivity, 90.5% specificity, and 93.75% accuracy.

CONCLUSION:
A negative ALK IHC result obviates the need for a FISH test barring those with a strong clinical profile, and a positive ALK IHC result is sufficient basis for the initiation of treatment.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Anaplastic lymphoma kinase
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Fluorescence in situ hybridisation
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Immunohistochemistry
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Lung adenocarcinoma
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Jambhekar NA
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Kumar R
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prabhash K
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Pramesh CS
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Desai SB
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Noronha V
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Karimundackal G
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Shah A
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Joshi A
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Laskar SG
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Jiwnani S
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Pai T
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Agarwal JP
773 ## - HOST ITEM ENTRY
Main entry heading Indian Journal of Cancer
Place, publisher, and date of publication Mumbai : Medknow Publications, 2017
Relationship information Vol. 54, no. 1, p. 148-154.
International Standard Serial Number 0019-509X
906 ## - LOCAL DATA ELEMENT F, LDF (RLIN)
Dept Pathology
Corporate name TMC
PMID 29199679
Article Type Journal Article
Country type National
Added Entry TMH
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Item type Articles
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent location Current location Date acquired Barcode Date last seen Koha item type
          Tata Memorial Hospital Tata Memorial Hospital 2017-12-21 AR18704 2017-12-21 Articles

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