Normal view MARC view ISBD view

A Comprehensive Serum Microrna Profiling in Indian Multiple Myeloma Patients Uniformly Treated With VCD-Protocol

In Clinical Lymphoma, Myeloma & Leukemia
By: Sriram H.
Contributor(s): Hasan S | Kedia S | Ghogale S | Deshpande N | Yajamanam B | Patkar N | Amare P | Inamdar N | Gokarn A | Punatar S | Bagal B | Jain H | Sengar M | Khattry N | Subramanian PG | Gujral S | Tembhare P.
Material type: materialTypeLabelArticlePublisher: 2019Description: .Subject(s): Multiple myeloma (MM) | Serum microRNA-profiling in India | VCD Protocol In: Clinical Lymphoma, Myeloma & Leukemia Vol. 19. no. (Suppl10), p. e80.Summary: Background & Introduction: Inadequate clinical response is a major cause of the poor clinical outcome in multiple myeloma (MM). Although many biomarkers are available for risk-stratification of MM, no markers are available that can reliably predict therapeutic response. Many studies have reported selective miRNA dysregulation at different stages of plasma-cell-neoplasms (MGUS to MM), and their association with high-risk MM. We have studied a comprehensive serum miRNA profiling in Indian MM patients treated with VCD-protocol to explore their clinical relevance in the prediction of initial response. Methods: Serum microRNA-profiling was performed in a discovery cohort of newly diagnosed MM patients and four age-matched healthy controls. Affymetrix miRNA 4.1 24-Array platform was used for expression profiling and datawas analyzed using GeneSpring. Results: Our study included 20 newly diagnosed MM patients (10 males and ten females) with agerange 35-75 years. A comprehensive miRNA-profiling revealed 13 miRNAs were differentially expressed between MM patients and healthy controls. Among these, ten miRNAs were significantly down-regulated, and three were up-regulated, with p-values<0.05. Down-regulated miRNAs include let-7a-5p, miR-23a-3p, miR150-5p, miR-320a, miR-342-3p, miR-320b, miR-320c, miR-320d, miR-4467 and miR-4485. Up-regulated miRNAs are miR-1281, miR-4440, miR-4801. Expression pattern of these 13 miRNAs divided MM patients into two clusters. However, the cohort of 20 was too small for any statistical correlation with the initial response. Hence, miRNA profiling is being performed in an additional 65 newly diagnosed MM patients. Conclusion: Our results revealed dysregulation of a new set of microRNAs as compared to reports published in western studies. It may be due to the ethnic variation of Indian patients from the western population. To validate our finding, we are studying the expression of these in an additional 65MM patient serum samples, and the results of the same will be presented in the meeting.
TMC TMH
Tags from this library: No tags from this library for this title. Add tag(s)
Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Call number Status Date due Barcode
Articles Articles Tata Memorial Hospital
Available AR19685

Abstract presented at 17th International Myeloma Workshop, Sept. 12-15, 2019

Background & Introduction: Inadequate clinical response is a
major cause of the poor clinical outcome in multiple myeloma
(MM). Although many biomarkers are available for risk-stratification of MM, no markers are available that can reliably predict therapeutic response. Many studies have reported selective miRNA dysregulation at different stages of plasma-cell-neoplasms (MGUS to MM), and their association with high-risk MM. We have studied a comprehensive serum miRNA profiling in Indian MM patients treated with VCD-protocol to explore their clinical relevance in the prediction of initial response.
Methods: Serum microRNA-profiling was performed in a discovery cohort of newly diagnosed MM patients and four age-matched healthy controls. Affymetrix miRNA 4.1 24-Array platform was used for expression profiling and datawas analyzed using GeneSpring. Results: Our study included 20
newly diagnosed MM patients (10 males and ten females) with agerange 35-75 years. A comprehensive miRNA-profiling revealed 13 miRNAs were differentially expressed between MM patients and healthy controls. Among these, ten miRNAs were significantly down-regulated, and three were up-regulated, with p-values<0.05. Down-regulated miRNAs include let-7a-5p, miR-23a-3p, miR150-5p, miR-320a, miR-342-3p, miR-320b, miR-320c, miR-320d, miR-4467 and miR-4485. Up-regulated miRNAs are miR-1281, miR-4440, miR-4801. Expression pattern of these 13 miRNAs divided MM patients into two clusters. However, the cohort of 20 was too small for any statistical correlation with the initial response. Hence, miRNA profiling is being performed in an additional 65 newly diagnosed MM patients. Conclusion: Our results revealed
dysregulation of a new set of microRNAs as compared to reports
published in western studies. It may be due to the ethnic variation of Indian patients from the western population. To validate our finding, we are studying the expression of these in an additional 65MM patient serum samples, and the results of the same will be presented in the meeting.

There are no comments for this item.

Log in to your account to post a comment.

Powered by Koha